Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.058096 |
Chromosome: | chromosome 14 |
Location: | 225459 |
Confidence (%): | 63 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g609050 | RPL34A | (1 of 4) 1.4.3.3 - D-amino-acid oxidase; Putative 80S Ribosomal protein L34-like protein | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGTGAGGGGACAGGCGTGCAGTGGTGGGGCACCTACCCCAGGGGCACA |
Internal bar code: | GCCTGGTCAGGGAGGGTGGCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2663 |
LEAP-Seq percent confirming: | 50.0 |
LEAP-Seq n confirming: | 48 |
LEAP-Seq n nonconfirming: | 48 |
LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGGTAGAGACCTGGTAGGGC |
Suggested primer 2: | CCTCGCCCTCAATACGTCTC |