| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.058100 |
| Chromosome: | chromosome 4 |
| Location: | 2959331 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224850 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGCGTGCGTACGTGCTTGCGCGATCGGTATCAGGAGGCAGCTGACGTG |
| Internal bar code: | TACAGCAAGTATTTATCTTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1685 |
| LEAP-Seq percent confirming: | 87.8049 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAATCCACCGCAGTTGGACC |
| Suggested primer 2: | CGTGTTGTCTGCGTGTTTGT |