| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.058106 |
| Chromosome: | chromosome 2 |
| Location: | 1348785 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082825 | TSS1,SERRS1 | Seryl tRNA(Sec) synthetase; (1 of 1) PTHR11778:SF0 - SERINE--TRNA LIGASE, MITOCHONDRIAL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTCTCATGCCTCCCCTCCCCTCCGTGCCAACCCCCCCCCCGCACCTG |
| Internal bar code: | CCATGTCGATGGACGGGTATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 743 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCCTTCTCCTTGCCTAC |
| Suggested primer 2: | GATTCAAGGGGTGGGGTGAG |