| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058107 |
| Chromosome: | chromosome 6 |
| Location: | 6610590 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g294650 | AGT1 | Alanine-glyoxylate transaminase; (1 of 2) K00830 - alanine-glyoxylate transaminase / serine-glyoxylate transaminase / serine-pyruvate transaminase (AGXT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGGCCTGACGGCGCGTAAGTCCCTACTCTAAGGGTAGCCCCAGCTCGT |
| Internal bar code: | AGCAGTATCTTAATGATCGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1274 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGATAGCGAGCTTAGCAA |
| Suggested primer 2: | TTGCTTGAGGACAGAGCCTG |