| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.058110 |
| Chromosome: | chromosome 8 |
| Location: | 3792274 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACTTCTTTGTGTGTCACCGCGGTACGCGCACACACACGGATCCACGGG |
| Internal bar code: | CGTAACGGTTCGTCCAGCTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3780 |
| LEAP-Seq percent confirming: | 63.6364 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCCTCGCCATTTGAAGAT |
| Suggested primer 2: | TGATTTCCGTGCAGTCTGCT |