Insertion junction: CLIP2.058117_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.058117
Chromosome:chromosome 10
Location:686763
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g422201 CTP4 Copper transporting ATPase; (1 of 2) K01533 - Cu2+-exporting ATPase (copB) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACTTCTCAGTTCTCGCGCCACCCCGAGCCACAACGACCGTCCCCTAAA
Internal bar code:GCCCGGTCGACAGCGACACTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1258
LEAP-Seq percent confirming:93.75
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTCTCTGACCTTGCTGGG
Suggested primer 2:ACACCCTCGCTGGATCTAGT