| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058121 |
| Chromosome: | chromosome 10 |
| Location: | 6538981 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465450 | (1 of 2) 2.8.2.20 - Protein-tyrosine sulfotransferase / Tyrosylprotein sulfotransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCACGGGCGACCATTCGGCCTGACCCCGCGATGCACCTGTATGCGTC |
| Internal bar code: | GTCCCATCAGGTTTTGGCTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2637 |
| LEAP-Seq percent confirming: | 36.3636 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTCTCTTAAGCCCGCGT |
| Suggested primer 2: | GTGTTGTGGCAAGCAGTGAG |