| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.058127 |
| Chromosome: | chromosome 10 |
| Location: | 942405 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424450 | TOM40 | 40 kDa translocon at mitochondrial outer envelope membrane; (1 of 1) K11518 - mitochondrial import receptor subunit TOM40 (TOM40) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACACGTCGCAGGTTGCCAAGTTGTGTGTTGCCGAGTGCAGAATTACAA |
| Internal bar code: | TCCGCCCCTAATTGCACTGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1389 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGGAGGAAGTGTGAGAA |
| Suggested primer 2: | CCACTGGCAGAGCAACTACA |