| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058129 |
| Chromosome: | chromosome 7 |
| Location: | 1433348 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g323450 | SMM24 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) 2.1.1.287 - 25S rRNA (adenine(645)-N(1))-methyltransferase / 25S rRNA m(1)A(645) methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGACGACCGAGGAATGCCGGTTTGGGAGCTGGTCAGCTGGCGGCTGC |
| Internal bar code: | TGGCAACACCAGAAGTATTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1158 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGGGAAGGACGAGACTC |
| Suggested primer 2: | AGGAAAACGTGTGTGGGTGT |