| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058131 |
| Chromosome: | chromosome 16 |
| Location: | 5514280 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682300 | RPS26 | (1 of 1) K02976 - small subunit ribosomal protein S26e (RP-S26e, RPS26); Cytosolic 80S ribosomal protein S26 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCTTAACATAAGCTGCTGCCATGTCTGGGCCAGCCAGCGCTGGCCGC |
| Internal bar code: | AAACATATGATGTCGAGCACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2671 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTCATTTTAGCGCGAGG |
| Suggested primer 2: | TGACGGAAGCTAAGCCCAAG |