Insertion junction: CLIP2.058135_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.058135
Chromosome:chromosome 3
Location:713223
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145887 TAT3,TATC,TATC1 (1 of 1) K03118 - sec-independent protein translocase protein TatC (tatC); TatC-like sec-independent protein translocon subunit 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCAAACCACAAAGCTGTACCCCCTGCACCAACCGTAACCTCTATCTCA
Internal bar code:GGTTAGGGGAACACGTGATCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:800
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACACACACACACCT
Suggested primer 2:CGCGTTGATGTGGTTTGTGT