| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.058138 |
| Chromosome: | chromosome 16 |
| Location: | 5204994 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684650 | (1 of 2) PTHR13748//PTHR13748:SF45 - COBW-RELATED // SUBFAMILY NOT NAMED; Putative metallochaperone | 5'UTR | |
| Cre16.g684700 | (1 of 1) PF10309 - Protein of unknown function (DUF2414) (DUF2414) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTTAAAGCAGCACATGCTTTAAAACGCAGTGGCATCGGCTGCTTCCAG |
| Internal bar code: | ATCCGTGCTTGAGCGTTGCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4146 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCACCTCGCCGAATTCA |
| Suggested primer 2: | AAACTTCTCCGCCCTGTCAG |