Insertion junction: CLIP2.058142_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.058142
Chromosome:chromosome 9
Location:5334135
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g407600 TEN1 Conserved TenA familiy member; (1 of 1) PTHR20858//PTHR20858:SF18 - PHOSPHOMETHYLPYRIMIDINE KINASE // PROTEIN PET18 3'UTR
Cre09.g407650 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTAACAAGGACGTGGCGTGACGTGCGCGGTGCGCCAAGTGCATGTAAGA
Internal bar code:CAGTAACGTCCCACTCCATTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1053
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGTCAAGAACACGGGCAC
Suggested primer 2:AAATGTGGCGTGACGTGTTG