| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058147 |
| Chromosome: | chromosome 15 |
| Location: | 5331563 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g734400 | CUL2,CUL3 | (1 of 1) K03347 - cullin 1 (CUL1, CDC53); Ubiquitin ligase SCF complex subunit Cullin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACACACACCGCAACTCCCGCGAGCGCGACCGCTCAAGCATTGCATA |
| Internal bar code: | GTCGGAGTACACTAGAGGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2964 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGACCCTGTTCAAGTACC |
| Suggested primer 2: | GGTTGACAAGGCTGCCAATG |