| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.058151 |
| Chromosome: | chromosome 2 |
| Location: | 3970977 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102551 | CSV1 | Chlamydomonas-specific family protein | 3'UTR |
| Cre02.g102600 | (1 of 1) PTHR23012:SF92 - RING/U-BOX DOMAIN-CONTAINING PROTEIN | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCTGTGCGTAGCGTCCATCGACGCACTGTTCGGTTAGATGCCGGAACA |
| Internal bar code: | GCTCAGAGCTTGTGGGGGACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 823 |
| LEAP-Seq percent confirming: | 45.8333 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGATGTCGTATGTTTGGCC |
| Suggested primer 2: | ATTGGAAGCGTTTTGGCACC |