| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.058160 |
| Chromosome: | chromosome 9 |
| Location: | 2044626 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396550 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTGACCGCCCGCCCCAAGTGTACCGCTGCTTGCGTCAGTCCGCGATG |
| Internal bar code: | TGTATTCGCTGATATATTAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2396 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCGAGTGACACGTTGGAA |
| Suggested primer 2: | TATCATCGTCGCCATCGTCC |