| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.058198 |
| Chromosome: | chromosome 10 |
| Location: | 5030500 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454734 | TIC21,PIC1 | (1 of 1) PTHR21649//PTHR21649:SF13//PTHR34548 - CHLOROPHYLL A/B BINDING PROTEIN // LHCA2 PROTEIN // FAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAACATTGCCAGCAACGATACAGGTTGATGCGGGTGTTGTATTGCGC |
| Internal bar code: | CCGCATTAATGGTCCCCTAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1341 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCTTCAGGCCTCTGCATT |
| Suggested primer 2: | GACATCGAGAACGTGGTGGT |