| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.058212 |
| Chromosome: | chromosome 3 |
| Location: | 5933909 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g188850 | (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain | 5'UTR | |
| Cre03.g188900 | (1 of 2) PF06127 - Protein of unknown function (DUF962) (DUF962) | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAAATGCTGCCGAGGACTGGCTGAATGGAATGCTAAGGCTCCATCCGG |
| Internal bar code: | CTTATTGTTGTTCGGTGGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3032 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGATAGACGAGGCAAGGC |
| Suggested primer 2: | GGTGTGTAAGGCGTGTGAGA |