| Insertion cassette: | CIB2 | 
| Side of cassette: | 3' | 
| Strand: | - | 
| Strain: | CLIP2.058223 | 
| Chromosome: | chromosome 6 | 
| Location: | 7194015 | 
| Confidence (%): | 80 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| Cre06.g298550 | FMO7 | Flavin-containing monooxygenase; (1 of 3) K00485 - dimethylaniline monooxygenase (N-oxide forming) (FMO) | 5'UTR | 
| Cre06.g298600 | (1 of 110) 3.4.19.12 - Ubiquitinyl hydrolase 1 / Ubiquitin thiolesterase | 3'UTR | 
| Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACTCCGGCAGCCAATTAACACATGTTTCAGACACACGACCGTTATCCT | 
| Internal bar code: | TGCAAGGCCAAATCGCGGGGGG | 
| Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2999 | 
| LEAP-Seq percent confirming: | 98.9796 | 
| LEAP-Seq n confirming: | 97 | 
| LEAP-Seq n nonconfirming: | 1 | 
| LEAP-Seq n unique pos: | 98 | 
| Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAACGCCAGCTACGGAAA | 
| Suggested primer 2: | AGCTAAGTTGCCATCGCTGT |