Insertion cassette: | CIB2 |
Side of cassette: | 5' truncated? |
Strand: | - |
Strain: | CLIP2.058227 |
Chromosome: | chromosome 7 |
Location: | 3977394 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g800824 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTAGGCTTGTGGCGTTGAGGCACAGCGGTGGGGCTAAGGCGGGTTGA |
Internal bar code: | CGCCCACCTAAAGCATCCTTTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1713 |
LEAP-Seq percent confirming: | 60.4651 |
LEAP-Seq n confirming: | 26 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAACATTGCAACTGCGCTGA |
Suggested primer 2: | CCTGCTCACTGCCTATACGG |