Insertion junction: CLIP2.058229_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.058229
Chromosome:chromosome 1
Location:5517206
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g039250 RPS2 Cytosolic 80S ribosomal protein S2; (1 of 1) K02981 - small subunit ribosomal protein S2e (RP-S2e, RPS2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATAGTTATGCTGTATTGAGTATAGACAGCGGGCACGTGATAGGGCTGTG
Internal bar code:TAGCTCTGTAACATGTCGGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2236
LEAP-Seq percent confirming:95.5556
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:GACTGTGCCAACCAGCTTTG
Suggested primer 2:GCCAGAAGCTGTGCAGACTA