Insertion junction: CLIP2.058229_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.058229
Chromosome:chromosome 1
Location:5517214
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g039250 RPS2 Cytosolic 80S ribosomal protein S2; (1 of 1) K02981 - small subunit ribosomal protein S2e (RP-S2e, RPS2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAACTATGCCGCTGCTGCCCCTTAGTTGGCAAAGCTGATTCAATGCATCG
Internal bar code:TAGCTCTGTAACATGTCGGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1300
LEAP-Seq percent confirming:29.4118
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GCCAGAAGCTGTGCAGACTA
Suggested primer 2:GACTGTGCCAACCAGCTTTG