| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058270 |
| Chromosome: | chromosome 6 |
| Location: | 1249794 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258650 | PGM6 | Phosphoglycerate mutase-like protein; (1 of 4) PTHR23029:SF1 - FRUCTOSE-2,6-BISPHOSPHATASE TIGAR | 3'UTR |
| Cre06.g258700 | PYC1 | (1 of 1) 6.4.1.1 - Pyruvate carboxylase / Pyruvic carboxylase; Pyruvate carboxylase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAAGTAGTTGTTTGCGCAGCGCGCAAGCGGTTCGAGGGGCAGCGTAAC |
| Internal bar code: | CCGAGTCACTCTAGTAGGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2220 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATTGTTACGGATGGCGGT |
| Suggested primer 2: | CGAATATTGCTGCAACCCGG |