| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058284 |
| Chromosome: | chromosome 12 |
| Location: | 5964311 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533950 | COT1 | Cobalt transport protein; (1 of 1) PTHR33514//PTHR33514:SF3 - FAMILY NOT NAMED // PROTEIN ABCI12, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAATACTTCAGTGGGCCGTCCCACAACCCACCGCCGACCATGCTTGG |
| Internal bar code: | GTTTGCTAGTTGTATACGTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3410 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGGTTGAGTGGTTGTGT |
| Suggested primer 2: | TAGGCTATGACGCAGCATGG |