Insertion junction: CLIP2.058292_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.058292
Chromosome:chromosome 17
Location:3266975
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g721900 COG5 Component of oligomeric golgi complex; (1 of 1) PF10392 - Golgi transport complex subunit 5 (COG5) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGCAATTGACGCGATGGGCAAAGGCGGTGATAGCACCGATGACAACCA
Internal bar code:CAACTTTAACTGCTGTAAGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1741
LEAP-Seq percent confirming:95.6522
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CGCAACTTCTGTGCAAGCTT
Suggested primer 2:AATCTGCAATCCGCTCACCA