| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.058305 |
| Chromosome: | chromosome 2 |
| Location: | 3947444 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102250 | RPS3 | Cytosolic 80S ribosomal protein S3; (1 of 1) K02985 - small subunit ribosomal protein S3e (RP-S3e, RPS3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTGAATCAAGTCGCTGTGGGTTTGCCGCGTAGGTACTGCACCTTAAGG |
| Internal bar code: | GCCTGGGATTGTCCCTACAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1800 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAAGCGCTTCTGCACAAC |
| Suggested primer 2: | GGCACCAACTACGAGGTCAA |