| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.058309 |
| Chromosome: | chromosome 7 |
| Location: | 5925984 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g354150 | MFT22,ARSJ1 | Major facilitator superfamily transporter; (1 of 82) IPR020846 - Major facilitator superfamily domain | 3'UTR |
| Cre07.g354200 | GAP2 | Glyceraldehyde 3-phosphate dehydrogenase, GAPC, cytosolic; (1 of 2) 1.2.1.12 - Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) / Triosephosphate dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCCTTCTGTATGTGCTCATGCTCATGCGTTCAGTTGCACCAATAAAA |
| Internal bar code: | ACCAGAGCGATTTCTCATTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2014 |
| LEAP-Seq percent confirming: | 94.6429 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTGTTGAGGTCGGGTTA |
| Suggested primer 2: | TCCCTCGTTCTTGTTCGGTG |