| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.058314 |
| Chromosome: | chromosome 3 |
| Location: | 833301 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146367 | (1 of 3) PTHR11697:SF74 - WITHOUT CHILDREN, ISOFORM B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCTATCCTGCTACGGGCACCCGCCTGTCCCATTCGCCTTGCCCGCGGC |
| Internal bar code: | TAGCACTATTACGTACTATACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3775 |
| LEAP-Seq percent confirming: | 23.75 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAACAGCTGTTCGCAAGC |
| Suggested primer 2: | ACTCGGACTTCAACGGGAAC |