Insertion junction: CLIP2.058324_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.058324
Chromosome:chromosome 6
Location:6269894
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g292400 SOUL5 (1 of 8) PF10184 - Uncharacterized conserved protein (DUF2358) (DUF2358); DUF2358 domain protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAAGATAAGCATCCAGTTACAGGTCGGGGCGCTGCGGGGGTGTCCCCAG
Internal bar code:TCGAATTGGCCTATGATCAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2056
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:TTCGGCTTCAGGTCTTGGTC
Suggested primer 2:TTTGTGTACGGACGCGAAGA