| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.058333 |
| Chromosome: | chromosome 12 |
| Location: | 2452340 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507558 | (1 of 1) 3.5.1.3 - Omega-amidase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCAGCTATGGAAATAAAGATGCTTGCAGTGACTATTACAACCGTGCC |
| Internal bar code: | GTAGAGAGTGTCATATCAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1491 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTCACAAGTTTGTCGCA |
| Suggested primer 2: | AGTTGAACATCTCGGGCAGG |