| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.058338 |
| Chromosome: | chromosome 12 |
| Location: | 8573786 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548700 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACCTCGCCTTGCTGGTCATTTGCTCCCACGCTCGCTTTCGCAACCCCC |
| Internal bar code: | CCGGTAAGTCCTTGGTACCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1823 |
| LEAP-Seq percent confirming: | 72.5 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACATGGAGAAGGGCCAG |
| Suggested primer 2: | TTCATCCGCCATTCCTTGCT |