Insertion junction: CLIP2.058344_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.058344
Chromosome:chromosome 17
Location:5622175
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g739950 (1 of 1) PF01612//PF13086//PF13087 - 3'-5' exonuclease (DNA_pol_A_exo1) // AAA domain (AAA_11) // AAA domain (AAA_12) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCACAAGCCTGGAGTCGCAAAACCCTATTACGGGACCACCTCGCTGCG
Internal bar code:ATGACATCGAGAGATTGTGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:4155
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:TGCACATGACAACAAGCGTG
Suggested primer 2:GTCGTGCTCACCACCTTGTA