| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.058356 |
| Chromosome: | chromosome 12 |
| Location: | 4882999 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523950 | (1 of 1) PTHR21229//PTHR21229:SF2 - LUNG SEVEN TRANSMEMBRANE RECEPTOR // PROTEIN C15H9.5 | 3'UTR | |
| Cre12.g801416 | (1 of 13) PF12847 - Methyltransferase domain (Methyltransf_18) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACCCGCAGCATGACAACAACATTCTCTCATTCGTTAAAGATTCCACC |
| Internal bar code: | AACCGAAGCCTGTAACGCCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1141 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTCAGCGCTTTTTGTGT |
| Suggested primer 2: | ACATCTCCTACCTCGCCCTT |