| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.058358 |
| Chromosome: | chromosome 12 |
| Location: | 2763221 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504150 | NFU2,NIFU2 | Iron-sulfur cluster assembly protein; (1 of 1) PTHR11178//PTHR11178:SF1 - IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED // NFU1 IRON-SULFUR CLUSTER SCAFFOLD HOMOLOG, MITOCHONDRIAL | 3'UTR |
| Cre12.g504200 | RPS23 | (1 of 1) K02973 - small subunit ribosomal protein S23e (RP-S23e, RPS23); Cytosolic 80S ribosomal protein S23 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCTGAATGAAAGGGCACGTAACCACCTCAACCGATCAGCCCATACGGC |
| Internal bar code: | TCTGGCAAGACGTTTGACGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1903 |
| LEAP-Seq percent confirming: | 96.9697 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCTGCAAGCCGATTTGA |
| Suggested primer 2: | TGTGTGCATACAGAGCAGCA |