| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.058360 |
| Chromosome: | chromosome 9 |
| Location: | 5877839 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g411900 | SHMT3 | (1 of 3) 2.1.2.1 - Glycine hydroxymethyltransferase / Threonine aldolase; Serine hydroxymethyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAATGACAGTTGCCGTACTTCTCGTTCCATCGTCGGAGAACTCTGTTT |
| Internal bar code: | GTTATTTTAAAGTGCGCTTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 952 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCTAGACACGACGGCCTG |
| Suggested primer 2: | GCAAGGTATGCGGCAACAAA |