| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.058368 |
| Chromosome: | chromosome 3 |
| Location: | 899189 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146567 | (1 of 781) IPR000104 - Antifreeze protein, type I | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCACAGCGAGGCTGGTTTCGAGGTCGGCAAGCTGTGGTGGTGGCATCG |
| Internal bar code: | TTGTGGCAGCGATAAATGTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1335 |
| LEAP-Seq percent confirming: | 38.8889 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCAATTGCAGCGGGTCTT |
| Suggested primer 2: | CGTGTTGCTCAGTTTGGTGG |