Insertion junction: CLIP2.058383_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.058383
Chromosome:plastome
Location:8967
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802265 ChreCp003,2716966,chlB (1 of 1) K04039 - light-independent protochlorophyllide reductase subunit B (chlB); light-independent protochlorophyllide reductase subunit B CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTAAATTTTGACAGTCAATAGAACGTGAAAACCAGGCTGCTTGGGAAA
Internal bar code:ATTCCGCCAAAGTTAATTATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:845
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TAGCCCGTCTAGCAAGTTGC
Suggested primer 2:AGTTTGTGACTGCCCGCTAG