| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.058401 |
| Chromosome: | chromosome 12 |
| Location: | 793950 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492200 | (1 of 6) 3.4.22.69 - SARS coronavirus main proteinase / Severe acute respiratory syndrome coronavirus main protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGGACGGCGAGGATGACGACACCGGCTCTCAGCAGCCGCGCGGGGTG |
| Internal bar code: | TAAGGTACGACTCGTTGGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 322 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGACAACAACTGCAGCCC |
| Suggested primer 2: | CTTCGCATGAATCAGCCGTG |