| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058406 |
| Chromosome: | chromosome 7 |
| Location: | 3206600 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g333950 | VPS45 | (1 of 1) K12479 - vacuolar protein sorting-associated protein 45 (VPS45); SM/Sec1-family protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGAAGTCAAAGTTTCCTATTTGACGAGCTTGCGCAGTATGGTAATCGT |
| Internal bar code: | TTGTAATTTTTACACAGGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1615 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGCAGACTGCCTGTCAT |
| Suggested primer 2: | TGATCGAAACCGTCCTGCAA |