Insertion junction: CLIP2.058410_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.058410
Chromosome:chromosome 7
Location:4712612
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g345250 (1 of 41) PTHR32523//PTHR32523:SF8 - FAMILY NOT NAMED // PHYTOL KINASE 1, CHLOROPLASTIC 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTTGGGGCTGCGAACTCCTGTTTGCAACATACTGATCGCTCAAATATG
Internal bar code:ATATGTTGCCAGTCGGGTTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1393
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CGGATCCTCGGTCAAGTACG
Suggested primer 2:GGGTGCCACAATTGCCAATT