| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058428 |
| Chromosome: | chromosome 13 |
| Location: | 1349921 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571450 | (1 of 2) K17278 - membrane-associated progesterone receptor component (PGRMC1_2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAAGGTTTTCAGCTAAGATACTGGCCATTTCGGCCTCCTCCTTGTCCG |
| Internal bar code: | GATTCGCTGAAGGACGGCTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4135 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCACCTTCTTGCAAGAAA |
| Suggested primer 2: | ACGCGCCTAAAGAGTATCGG |