| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.058442 |
| Chromosome: | chromosome 14 |
| Location: | 3897346 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632759 | (1 of 2) 4.3.1.19 - Threonine ammonia-lyase / Threonine dehydratase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGTTTGGGGCTTGCAAATGTACAAACTGATGTCAGCGGTTTGCTGTG |
| Internal bar code: | AGCGGTCTCATCACTGACCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2216 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGCAAGGTCTCTGATGC |
| Suggested primer 2: | CGTAGCTAGGTAGGAAGCGC |