Insertion junction: CLIP2.058474_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.058474
Chromosome:chromosome 1
Location:4311005
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g029300 TPI1,TPIC1,TPIC (1 of 1) 5.3.1.1 - Triose-phosphate isomerase / Triosephosphate mutase; Triose phosphate isomerase, chloroplast precursor 5'UTR
Cre01.g029350 C1d-87,FAP297 Flagellar central pair-associated protein 297; (1 of 1) IPR006885//IPR017986 - NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial // WD40-repeat-containing domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAAGTCCGCCCACCAGCCGTCTGTGCTACGCTACACCTCTGCACTGCT
Internal bar code:GAGATCTTATAATCGTCCGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:870
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CAATGCAGGCGATTACACCG
Suggested primer 2:AGAGTGAAGCGACAGTTGGG