| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.058479 |
| Chromosome: | chromosome 12 |
| Location: | 762846 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492500 | (1 of 39) IPR009003 - Peptidase S1, PA clan | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACACGAGCCCGCTCTAGGTCATTCGCCATGCTCGCAACGCCCGAAGGC |
| Internal bar code: | GCGAAGCGCTCGGATCATGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 517 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGCTGCAATTCCCGATTG |
| Suggested primer 2: | CACACACACGAGAGCTAGGG |