| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058484 |
| Chromosome: | chromosome 6 |
| Location: | 646767 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g252650 | LEUS1,IPMI1,LEU1S | (1 of 1) K01704 - 3-isopropylmalate/(R)-2-methylmalate dehydratase small subunit (leuD); Isopropylmalate dehydratase, small subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCCGGCACAACTCGCTTTACATAATCAGCAATAAAAACCATCGGCAAG |
| Internal bar code: | GGTGGGTAGCTGGGAAAGCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3420 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 155 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 155 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCTTACAGGCTCGGAGGT |
| Suggested primer 2: | GCAACCGCAGAGAAAATGCA |