| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.058501 |
| Chromosome: | chromosome 4 |
| Location: | 3743978 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g229374 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCGCCCACACAGCTGACGCCGCCGCAGCCAGGCGTCAACCCCGTCGA |
| Internal bar code: | ATATGTCATTATTGTTAGCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2410 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 90 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGACTACGGTTTCCACGG |
| Suggested primer 2: | CTCTCTGGCTCTGAGACCCT |