Insertion junction: CLIP2.058507_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.058507
Chromosome:chromosome 2
Location:5898778
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g117550 ANK13 (1 of 1) K06694 - 26S proteasome non-ATPase regulatory subunit 10 (PSMD10); Predicted protein with ankyrin repeats 3'UTR
Cre02.g117600 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTAACGGACACACTCGGACAACTGCGCCCACAGTGAGGTAATTGTAGG
Internal bar code:TATCGTCCTAGTTATTGGTCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1572
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGGGATGGGGATTGGGAT
Suggested primer 2:TGGAAGCAAGCTGGTGTTGA