| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.058508 |
| Chromosome: | chromosome 11 |
| Location: | 2510509 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095104 | (1 of 1) K15193 - protein SPT2 (SPTY2D1, SPT2) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGGAGGAGTTGGGCCGTGTTTAGGTTAGCTGGGGCTGCGGGGCTGCGG |
| Internal bar code: | CTTTTATCTTAGTCGCTCGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1891 |
| LEAP-Seq percent confirming: | 98.7952 |
| LEAP-Seq n confirming: | 82 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTAGTCCACCAGTGCTG |
| Suggested primer 2: | AGAGGGGCTAGCTGCTTTTG |