Insertion junction: CLIP2.058510_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.058510
Chromosome:chromosome 15
Location:3712909
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre15.g801809 (1 of 30) PF00078//PF03372 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) // Endonuclease/Exonuclease/phosphatase family (Exo_endo_phos) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGTGTGCGTATGTCGTTTTCCGTGTAGGGCATGACGGCGAAACTATATG
Internal bar code:AAGTGGTCCACCTGGTGACCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2359
LEAP-Seq percent confirming:1.25
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:79
LEAP-Seq n unique pos:80

Suggested primers for confirmation by PCR

Suggested primer 1:TACACGCACTTGAAGACGCT
Suggested primer 2:ACTCGGTGCTTATGTTGGCA