Insertion junction: CLIP2.058534_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.058534
Chromosome:chromosome 11
Location:358813
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467577 (1 of 2) PF00319 - SRF-type transcription factor (DNA-binding and dimerisation domain) (SRF-TF) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCAAGGTCGCTGGCGGGGCTGTCTGCACCCGCCCTCCATCATTGCGCT
Internal bar code:AATACCGTCTGGGGGAGGCGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3036
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GCACAACGAGAGGTCAAGGA
Suggested primer 2:GTCCGTGTCCAGCCCATAAA